PCR Biases Distort Bacterial and Archaeal Community Structure in Pyrosequencing Datasets
Figure 2
The taxa detection frequency for each of the replicate mock communities at different sequencing depths are compared to detection frequency at different theoretical sampling depths.
Open circles: sub-samples of in-silico mock communities with varying number of sequences, red circles: large library, green circles: small library, solid lines: 95% confidence interval band for the in-silico sub-sampling efforts. A–C: bacteria, D–F: archaea, A/D: mock1, B/E: mock 2, C/F: mock 3. Theoretical taxa detection frequencies for mock community 1 (bacteria and archaea) are 1.0 for most in-silico sub-sampling efforts and hence are not shown in panels A and D.