Binding of Translationally Controlled Tumour Protein to the N-Terminal Domain of HDM2 Is Inhibited by Nutlin-3
Figure 2
Inhibition of TCTP-HDM2 interaction by Nutlin-3 and p53 peptide.
A, In vitro translated HDM2 was immobilised on beads and pre-treated with indicated amounts of Nutlin-3 or p53 peptide/p53 control peptide (1 mM). Bound TCTP detected by Western blot (anti-FLAG antibody). Control lane indicates TCTP pull-down in the absence of HDM2. B, Recombinant HDM2 (residues 17–125) or control peptide (GS-control) was immobilised on beads and incubated with recombinant TCTP either in presence or absence of 200 µM Nutlin-3.