Gene Expression Signatures of Extracellular Matrix and Growth Factors during Embryonic Stem Cell Differentiation
Figure 6
Gene networks identified during early EB differentiation.
Network analysis using Ingenuity Pathway Analysis software was performed on genes that changed significantly over the course of differentiation and that exhibited at least a two-fold expression change compared to ESCs. The shift in the top networks between (A) day 4, (B) day 7, (C) day 10, and (D) day 14 demonstrates a progression from a more undifferentiated state (A) towards more differentiated ones (B–D). Relationships are divided into direct (solid line) and indirect (dashed line, - - - -) interactions, with arrow heads exhibiting the affected gene, as well as several “hub” genes. Varying intensities of green denote negative fold changes, while shades of red indicate positive fold changes. Grey genes represent genes with fold changes less than 2 that are related to other genes in the network, while genes with no color were not contained in the array data set.