Yeasts Acquire Resistance Secondary to Antifungal Drug Treatment by Adaptive Mutagenesis
Figure 6
Follow up of S. cerevisiae microcolonies during prolonged exposure to 5-FC.
Follow up of S. cerevisiae microcolonies during prolonged exposure to 5-FC for the specified time. After inoculating a 90 mm diameter Petri dish containing SC medium with 100 µg/ml 5-FC in the same fashion as performed for SLAM experiments, a 6 cm2 slice was cut out and placed on a glass slide and kept in a moist chamber. Random fields were chosen at time 0 and followed through time as specified in the Methods section for the times indicated in each picture. Panel A shows detailed follow up of one field. Adjacent to the field shown in panel B, a cell acquired resistance and grew, eventually invading the followed up field (black arrow). A marked difference can be observed between the edge of microcolonies and the 5-FCr colony.