Contribution of the Staphylococcus aureus Atl AM and GL Murein Hydrolase Activities in Cell Division, Autolysis, and Biofilm Formation
Figure 2
Growth characteristics of the atl mutants.
A) UAMS-1 (black solid diamonds), Δatl mutant KB5000 (red empty circles), KB5000 with pJB141 (red solid circles), ΔatlAM mutant KB5002 (green empty square), KB5002 with pJB111 (green solid square), ΔatlGL mutant KB5001 (blue empty triangle), and KB5001 with pJB135 (blue solid triangle) were grown in TSB with a 1∶10 media to volume ratio at 37°C with shaking (250 rpm). Data represent the mean (n = 2) with standard error. B) Relative cluster size as determined by flow cytometry of mid-exponential phase UAMS-1 (black), Δatl mutant KB5000 (red), ΔatlAM mutant KB5002 (green), and ΔatlGL mutant KB5001 (blue). UAMS-1 and mutants contain the control vector, pJB128.