Aromatase Inhibition Attenuates Desflurane-Induced Preconditioning against Acute Myocardial Infarction in Male Mouse Heart In Vivo
Figure 5
Aromatase expression in endothelial and smooth muscle cells.
Aromatase cellular localisation pattern in endothelial (EC) and smooth muscle cells (SMC) was assessed by immunocytochemistry and visualized using confocal imaging technique. Aromatase was immunostained using specific primary antibody and visualized by addition of the secondary antibody conjugated with AlexaFluor594 fluorophore (red). Aromatase was abundant in the cytosolic fraction of endothelial (A) and smooth muscle cells (C). Cell nuclei were detected using lamin A/C antibody and visualized using AlexaFluor488- labelled secondary antibody (green). Merge images B and D show aromatase and lamin A/C co- immunostaining.