Germ Cell Development in the Scleractinian Coral Euphyllia ancora (Cnidaria, Anthozoa)
Figure 7
Characterization of anti-Eavas immunoreactivity in male colonies.
Immunohistochemical analysis with the anti-Eavas antibody was conducted to examine Eavas immunoreactivity (irEavas) in cells at different stages of spermatogenesis. A. irEavas-positive spermatogonia located along the mesenterial mesoglea of a coral sample harvested in August (stage 0). B. A higher magnification view of the inset shown in A. Arrowheads indicate spermatogonia that display darker Eavas staining than others. C. Spermatogonia located along the mesenterial mesoglea of a coral sample harvested in October (late stage 0). The arrows indicate small spermatogonial clusters. D. A higher magnification view of the inset shown in C. The arrow indicates a spermatogonial cluster. The arrowheads indicate spermatogonia that exhibit darker irEavas staining compared with other spermatogonia. E. irEavas-positive spermatogonia located in the mesentery of a coral sampled in December (stage I). The arrows indicate spermatogonial clusters. F. A higher magnification view of the inset shown in E. G. irEavas stained spermatocytes located in the mesentery of a coral sampled in March (stage II). The arrows indicate the clusters within the mesoglea. H. A higher magnification view of the inset shown in G. I. Immunohistochemical analysis with the anti-Eavas antibody to examine Eavas immunoreactivity in stage III and IV male germ cells that were collected in April. J. Immunohistochemical analysis of stage V male germ cells collected in May. III, stage III; IV, stage IV; V, stage V. K. Immunohistochemical characterization of the anti-Eavas antibody using stage I male germ cells. L. The anti-Eavas antibody was preadsorbed with the peptide antigen. The preadsorbed control sample was nearly devoid of immunoreactivity. The arrow points to a cluster of male germ cells. The scale bars correspond to 50 µm in panels A, C, E, G, I and J, and 10 µm in panels B, D, F, H, K and L.