Identification of CBX3 and ABCA5 as Putative Biomarkers for Tumor Stem Cells in Osteosarcoma
Figure 4
Differential transcript and protein expression in monolayers and spheres.
(A) Nestin (NES) transcript in CHA59 monolayers and spheres measured by microarray analysis, * p<0.05. (B) Nestin (NES), (C) vimetin (VIME), and (D) chromobox protein homolog 3 (CBX3) protein expression in CHA59, Saos-2, and HuO9 cells tested by western blotting. ‘M’ represents monolayers, ‘S’ represents spheres. (E) Immunophenotype of monolayers and spheres probed by flow cytometry. CHA59 and Saos-2 immunophenotype reported as a cumulative of 6-color panel (CD326, CD166, CD133, CD24, CD44, excludes ABCB1) and 3-color panel (ABCB1, ABCC1, ABCG2). HuO9 immunophenotype reported as a cumulative of 6-color panel (CD326, CD166, CD133, CD24, CD44, ABCB1) and 2-color panel (ABCC1, ABCG2). p value calculated for monolayers vs. spheres for each cell line. ‘M’ represents monolayers, ‘S’ represents spheres.