Identification of CBX3 and ABCA5 as Putative Biomarkers for Tumor Stem Cells in Osteosarcoma
Figure 1
Spheres demonstrated increased clonogenicity and tumorigenicity compared with monolayers.
(A) CHA59, Saos-2, and HuO9 monolayers generated spheres in FBS-free, SS media. Phase contrast images taken with a 40× objective. (B) CHA59 spheres formed colonies in both FBS-A (Fetal Bovine Serum-Agarose) and SS-A (KnockOut Serum Replacement-Agarose). Images taken with a 10× objective. C) CHA59 spheres demonstrated significantly higher clonogenicity as compared with monolayers in FBS-A and SS-A, * p<0.05. (D) CHA59 Spheres demonstrated higher tumorigenicity than monolayers. 10 NOD/SCID mice injected with cells per group. A xenograft of ≥150 mg was counted positive for take rate.