Identification and Characterization of the Cognate Anti-Sigma Factor and Specific Promoter Elements of a T. tengcongensis ECF Sigma Factor
Figure 5
The effects of truncation in the −35 and −10 regions on the interaction between Tt-RpoE1 and promoter DNA.
(A). EMSA results of truncation in the −35 region. The structure of parental probe is provided at the top, and vertical lines indicate truncated positions in the double-stranded region. Both the top and bottom strands were truncated from −40 to different positions indicated at the left of the fork-junction probe. The 3′ terminus of top strand was kept at +1, and the 5′ terminus of the bottom strand was kept at −10. (B). EMSA results of truncations in the −10 region. The structure of parental probe is provided at the top. The T+1 was the top strand for all the probes, and the bottom strand was truncated from B+1 to different positions as indicated on the right of the bottom strand. The dots denote the terminal bases in the bottom strands in fork-junction probes. The protein concentration was 5 µM in all of the following experiments.