Estrogen Receptor β Activation Impairs Prostatic Regeneration by Inducing Apoptosis in Murine and Human Stem/Progenitor Enriched Cell Populations
Figure 3
ERβ induces apoptosis in regenerative murine and human prostatic basal cells.
(A) Representative flow cytometry plots showing live, dead and apoptotic cells following vehicle or 8β-VE2 treatment of digested mouse prostate. (B) Percent apoptotic cells following vehicle (grey bar) or 8β-VE2 (open bar) treatment of digested mouse prostate (n = 3 with 3 mice/group/experiment). (C) Representative flow cytometry plots showing gating for regenerative murine LSC cells. (D) Changes in the regenerative murine LSC population following vehicle (grey bar) or 8β-VE2 treatment (open bar) (n = 3 with 3 mice/group). (E) Representative flow cytometry plots following vehicle or 8β-VE2 treatment of human prostatic epithelial cells. (F) Changes in the regenerative CD49fhiTrop2hi population following vehicle (grey bars) or 8β-VE2 treatment (open bars). (n = 3 patients), **P<0.01 by students t-test.