Mycobacterium tuberculosis Infection of Dendritic Cells Leads to Partially Caspase-1/11-Independent IL-1β and IL-18 Secretion but Not to Pyroptosis
Figure 6
Highly purified CD11c+ dendritic cells confirmed the partially caspase-1/11 independent inflammasome activation upon infection with Mtb.
CD11c+ sorted BMDCs from wild-type WT and Casp1/11−/− were left uninfected (UI, black bars), infected with Mtb (white bars) or the ΔesxA mutant (gray bars). After 24h the percentage of TUNEL positive cells was determined in (A). In (B) the amount of necrosis was determined by quantification of the release of adenylate kinase and normalized to the values obtained in detergent lysed cells (PC, striped bar). In (C) and (D) the same supernatants were analyzed for IL-1β and IL-18 secretion, respectively.