GmcA Is a Putative Glucose-Methanol-Choline Oxidoreductase Required for the Induction of Asexual Development in Aspergillus nidulans
Figure 8
Nitrogen and carbon regulation of brlA expression in the ΔgmcA mutant.
A) Northern blot experiments showing brlA expression in wild type (TN02A3) and ΔgmcA (BD429) genetic backgrounds during vegetative growth (18 hours), labeled as 0, and 6, 12 and 24 hours after the induction of asexual development. RNA samples were obtained as described in Materials and Methods. Nitrate (80 mM) was used as the main nitrogen source while glucose (Glu) or glycerol (Gly) was used as carbon source. B) gmcA and brlA expression levels in wild type (TN02A3), ΔflbB (BD177), ΔgmcA (BD429) and double null (ΔflbB, ΔgmcA; BD431) backgrounds, at the same time points as in panel A. RNA samples were obtained from mycelia grown in nitrate (80 mM) or ammonium (10 mM)-containing media, using glucose (1%) as the main carbon source. rRNA: Ribosomal RNAs, used as loading control.