First Human Model of In Vitro Candida albicans Persistence within Granuloma for the Reliable Study of Host-Fungi Interactions
Figure 2
Fungal granuloma progression by scanning electron microscopy.
Kinetics of granuloma formation after infection with C. albicans (MOI of 2000∶1). The granuloma structures were collected at different time points from co-culture plates, and fixed in 2.5% glutaraldehyde, O.1 M sucrose in cacodylate buffer for 1 h. After dehydratation through a gradient ethanol series and alcohol-freon substitution, specimens were coated with 100 Å of gold-palladium mix in an ion sputter and photographed under a scanning electron microscope. Bars represent 5 µm (Days 1 post-infection), 10 µm (days 2 and 4) and 50 µm (day 6). Activated macrophages (Mf), lymphocytes (Ly).