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Achieving λ/10 Resolution CW STED Nanoscopy with a Ti:Sapphire Oscillator

Figure 6

Confocal and corresponding CW-STED images of actin filaments in a HeLa cell.

(a) Confocal; (b) CW-STED. The boxed regions in (a) and (b) are magnified in (c) and (d), respectively. The intensities at the cross-section marked in (c) and (d) are plotted in (e). The wavelength and power density for the STED beam is 763 nm and ∼127 MW/cm2. Scale bar: 500 nm.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0040003.g006