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Linear Fidelity in Quantification of Anti-Viral CD8+ T Cells

Figure 4

Direct quantification of HSV-specific CD8+ T cells.

Splenocytes from mice infected with HSV KOS were serially diluted in splenocytes from mice infected with HSV K.L8A and CD8+ T cell responses to the peptides gB498 and RR1982 were measured using three methods. (A) Data from DimerX, IFNγ-ICS and IFNγ-ICS/CD107 methods as indicated on each graph. Backgrounds determined using irrelevant peptides were subtracted from the values presented. (B) Linear regression analysis and r2 statistics for quantification of gB498–specific CD8+ T cells by each method.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0039533.g004