Effects of fou8/fry1 Mutation on Sulfur Metabolism: Is Decreased Internal Sulfate the Trigger of Sulfate Starvation Response?
Figure 10
Sulfate uptake and flux through sulfate assimilation in fou8 and related mutants.
WT Col-0 and mutants fou8, fou2, and aos were grown for 3 weeks on MS-phytagel vertical plates in controlled environment room. The seedlings were incubated for four hours with their roots submerged in nutrient solution adjusted to sulfate concentration of 0.2 mM and supplemented with 6.7 μCi [35S]sulfate. Shoot and root material was harvested separately, and the flux was determined as incorporation of 35S from [35S] sulfate to thiols and proteins. A sulfate uptake, B Percentage of 35S transported to leaves from the [35S]sulfate taken up, C relative flux through the sulfate assimilation in the leaves calculated as % of incorporation in thiols and proteins from total [35S]sulfate taken up. Results are presented as means ± SE from six independent pools of 8 seedlings grown in two independent experiments. Values marked with an asterisk show significant (P≤0.05) difference from Col-0.