Modulation of Lactobacillus plantarum Gastrointestinal Robustness by Fermentation Conditions Enables Identification of Bacterial Robustness Markers
Figure 7
Relative GI-tract survival of L. plantarum mutants with cat replacements of candidate genes involved in GI-tract survival.
Log10 CFU ml−1 determination of logarithmic (OD600 = 1.0) batch L. plantarum mutants after 60 min gastric juice incubation (white bars) and subsequent 60 min pancreatic juice incubation (grey bars). Input is set at 0 Log10 CFU ml−1. Δpbp2A::cat = L. plantarum NZ3412CM, Δ1669::cat = L. plantarum NZ3417CM, Δ1817::cat = L. plantarum NZ3414CM, ΔpacL3::cat = L. plantarum NZ3415CM, and ΔnapA3::cat = L. plantarum NZ3416CM. * P-value <0.05, ** P-value <0.01 compared with wild type (wt). Representative of two independent experiments, data presented are averages of technical sextuplicates (+ standard deviation).