Skipping of Exons by Premature Termination of Transcription and Alternative Splicing within Intron-5 of the Sheep SCF Gene: A Novel Splice Variant
Figure 2
Sequencing chromatogram of cDNA in comparison with the gDNA amplification of oSCF gene.
(a) Complementary DNA (cDNA) chromatogram shows the CDS, GT repeats and p(A)18 tail adapter primer as ‘black dotted oval mark’ and a premature termination codon (PTC) as ‘red dotted oval mark’ on the 3′ RACE product (336 bp, see Figure 1B(b5)); (b) Genomic DNA (gDNA) chromatogram shows the counter part of the above cDNA illustration (a) on exon 5 to exon 6 intervened by intron-5 sequences of the oSCF gene; (c) Amplification scheme of 948 bp splice junction covering exon(5)-intron-5-exon(6) of the oSCF gene with reference to human and mouse. The two exons 5, 6 are differentiated by ‘open and shaded box’ respectively. Arrows over the boxes indicate the fwd and rev primer. Different symbols on the intron-5 indicate the part of retained intronic sequences (161 bp) by a PTC along with the stretch of p(A)11 signal (see key to symbols below the diagram); (d) Gel picture shows the PCR amplification of 948 bp fragment corresponding to the above schema (c) of oSCF gene from blood gDNA; In the picture, arrow mark indicates the exact size of amplicon; M1 indicates DNA size marker of λ-DNA EcoRI/HindIII digest; and (−)ve represents PCR negative control.