The Epitope and Neutralization Mechanism of AVFluIgG01, a Broad-Reactive Human Monoclonal Antibody against H5N1 Influenza Virus
Figure 7
Inhibition of AVFluIgG01 on HA-mediated cell-cell fusion.
A. AVFluIgG01 inhibits the low-pH induced cell-cell fusion in Vero cells that were transfected with the plasmid encoding Viet04 HA protein (pViet04-HA). The cells were stained with crystal violet. (a) Control cells (no HA transfection); (b) Positive control (without antibodies); (c) Treated with 50 µg/ml AVFluIgG01; (d) Treated with 50 µg/ml control mAb (AVFluIgG03). B. AVFluIgG01 inhibits cell fusion between 293T effector cells and MDCK target cells at a dose-dependent manner. 293T cells were co-transfected with pViet04-HA and pGAL4-VP16 and MDCK cells were transfected with pGal5-luc. After fusion induced by low-pH, the luciferase activity was measured in the cell lysates. The percent inhibition and IC50 were calculated.