Artificial Polyploidy Improves Bacterial Single Cell Genome Recovery
Figure 2
B. subtilis response to PC190723 treatment: Increased DNA content.
DNA content was assessed for PC190723 treated (maximum effect from ten replicates) and control cells via six replicates of 50 sorted cells for primer sets A (located near the origin) and B (located near the terminus). Theta or multi-fork replication may account for the discrepant reports from both primer sets. Cells treated for 50 minutes had more DNA content than the respective untreated controls. Prolonged drug exposure may account for the reducing DNA content at 60-min treatment as related to DNA degradation. Error bars are 95% CI.