Cataract-Causing Defect of a Mutant γ-Crystallin Proceeds through an Aggregation Pathway Which Bypasses Recognition by the α-Crystallin Chaperone
Figure 5
Western Blot analysis of I90F + αB native mixing.
(A) Size exclusion chromatograms of the I90F + αB mixture after a 27 day incubation at 37°C. The shaded areas represent the fractions that were analyzed by Western Blot. (B) Two Western Blots were performed on identical sets of samples. The upper panel detected the presence of αB and the lower panel detected HγD. Numbers along the bottom are SEC fractions. Fractions 13–16 comprised the complex peak; fractions 23–25 comprised the αB peak; fractions 35–37 comprised the I90F peak.