Identification of miRNA Signatures during the Differentiation of hESCs into Retinal Pigment Epithelial Cells
Figure 2
Select miRNAs are dynamically expressed during differentiation of H9 ES cell line.
Representative miRNA expressions that are A) down-regulated or B) up-regulated during the course of differentiating H9 ESCs. All values were normalized to ES state, then log2 transformed. C) RT-PCR analysis of different RPE differentiation stage showing miR-23a, miR125a, miR-125b, miR-302d and let-7g expression relative to H9 ESC stage. The relative expression levels for each miRNA were normalized by hsa-U6 control. PD = partially differentiated, PC = pigmented clusters, ES = embryonic stem cells.