Human FOXN1-Deficiency Is Associated with αβ Double-Negative and FoxP3+ T-Cell Expansions That Are Distinctly Modulated upon Thymic Transplantation
Figure 3
Reduced but functional CD8+ T-cell compartment after fully-mismatched HLA class I thymus transplantation.
(A) Circulating CD8+ αβ T-cells analysed 58 months post-transplantation (5% of total lymphocytes; 83cells/µl) by flow cytometry in terms of: naïve/memory/effector phenotype assessed by CD45RA and CD27 expression; levels of activation markers (CD38 and HLA-DR); IFN-γ and IL-2 production upon PMA+ionomycin stimulation; ex vivo frequency of cycling cells (Ki-67+); and ex vivo frequency of apoptotic cells assessed by annexin V staining. (B) TCR repertoire diversity evaluated by spectratype of CDR3 Vβ regions of purified CD8+ T-cells at 59 months post-transplant. (C) Graph shows the coordinate increase in the proportion of terminally differentiated effector cells defined as CD45RA+CD27neg cells within total CD8+ T-cells during acute varicella infection and its decrease in parallel with its clinical resolution.