Ventx Factors Function as Nanog-Like Guardians of Developmental Potential in Xenopus
Figure 5
mNanog expression rescues specifically ventx1/2 morphant embryos.
(A) Two-cell stage embryos (NF2) were first injected radially twice with control MO (30 ng/blastomere), or a 1∶1 mix of ventx1 and ventx2 MOs (ventx1/2 MOs; 30 ng/blastomere), and subsequently injected radially at NF3 in all blastomeres with mNanog mRNA (0.15 ng/blastomere), msx1 mRNA (0.15 ng/blastomere), or with water. (B) Range of phenotypes observed in rescue of ventx1/2 knockdown experiment. (C) Percentages observed for each phenotypic category in three independent replicates of the rescue experiment. The combined injections performed are indicated at the bottom of the graph, and the number of injected embryos for each condition is indicated on the top of each bar. NF28 embryos were processed for WISH with six6, egr2 and hoxb9 (D), or six6, shh and hba4 (E) probes (anterior to the left, dorsal to the top).