Activation of Pax7-Positive Cells in a Non-Contractile Tissue Contributes to Regeneration of Myogenic Tissues in the Electric Fish S. macrurus
Figure 10
Intracellular tracer dye injections into single muscle fibers and electrocytes at the level of the distal-most ventral fin reveal no cell fragmentation.
Fluororuby (red) was injected into single electrocytes (A) and muscle fibers (B) and visualized 7 days after injection by retracting the overlying skin of the fish. Tail sections at the level of the distal-most region of the ventral fin were processed for immunolabeling and viewed under a fluorescent microscope. (C), region of a cryosection containing muscle fibers injected with Fluororuby dextran (red) and counterstained with DAPI (blue). (D), same image as C showing only DAPI labeling. Scale bars: A and B, 20 µm; C and D, 10 µm.