Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis
Figure 9
Expression of cathepsin B and cystatin B in the basal ganglia of post-mortem brain tissues.
Basal ganglia tissue from HIV-seronegative (A, F and K) and HIV seropositive (B-E, G-J and L-O) patients were stained with mouse anti-human cathepsin B followed by Alexa 488 conjugate goat anti-mouse (green), or mouse anti-human cystatin B followed by Alexa 488 conjugate goat anti-mouse (green), and rabbit anti-human Iba-1 followed by Alexa 546 goat anti-rabbit (red), and nuclear staining by DAPI (blue). Unstained tissues were used as controls as illustrated in K to O. Magnification: 63×.