Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis
Figure 8
Expression of cathepsin B and cystatin B in the hippocampus of post-mortem brain tissues.
Hippocampal tissue from HIV-seronegative (A, F and K) and HIV-seropositive (B-E, G-J and L-O) patients were stained with mouse anti-human cathepsin B followed by Alexa 488 conjugate goat anti-mouse (green), or mouse anti-human cystatin B followed by Alexa 488 conjugate goat anti-mouse (green), and rabbit anti-human Iba-1 followed by Alexa 546 goat anti-rabbit (red), and nuclear staining by DAPI (blue). Hippocampus samples from the same patients stained only with secondary antibodies and DAPI were used as negative controls illustrated in K-O. For each color, detector gains were maintained standard in every caption taken with the Pascal software in a Zeiss LSM 5 confocal laser-scanning microscope using a 63× magnification.