p53 Activation following Rift Valley Fever Virus Infection Contributes to Cell Death and Viral Production
Figure 1
p53 is phosphorylated on several residues following infection with RVFV.
A) HSAECs were either mock or MP-12 infected at an MOI of 3.0. Cells were collected 6, 24 and 48 hours post infection and lysates were analyzed by western blotting for antibodies against p53 at the residues indicated (Ser 9, Ser 20, Ser 37, Ser 46 and Ser 15). B) HSAECs infected in the same manner as Fig. 1A were collected at the time points indicated and analyzed by western blotting for antibodies against total p53 and p53 (Ser392). C) HSAECs were treated in the same manner as Fig. 1A and Fig. 1B and analyzed by western blotting for antibodies against the N protein of RVFV. D) HSAECs were infected with UV inactivated MP-12 (MOI 3.0) and collected at 24 and 48 hours post-infection. In parallel, HSAECs were treated with doxorubicin (1 µM) and collected at 24 hours. Cell lysates were analyzed by western blot analysis as describe in panels A and B. Actin is used as a loading control in all panels.