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Complex Interactions between Human Myoblasts and the Surrounding 3D Fibrin-Based Matrix

Figure 2

Localization and expression of the adhesion molecules in the 3D constructs.

Immunofluorescence staining of myoblasts in 3D fibrin constructs or on routine 2D surface (panel A). Scale bar: 10 µm. Representative Western blots of α5 integrin (α5 int), vinculin (vinc), FAK and actin in 3D (first lanes) and 2D (second lanes) cultures (panel B). Quantification of the Western blot was performed using lamin A as a loading control and expressed in arbitrary units (panel C). Values are means ± SD, n = 4; * p<0.05, *** p<0.001 vs value in 2D.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0036173.g002