JNK3 Maintains Expression of the Insulin Receptor Substrate 2 (IRS2) in Insulin-Secreting Cells: Functional Consequences for Insulin Signaling
Figure 3
Effect of JNK silencing on downstream substrates of insulin signaling.
Cells were transfected with Jnks (si1, si2, si3) or GFP (siGFP) siRNAs for 2 days and then exposed to cytokines (4 hrs). Western blot analysis was performed to determine (A) phospho-GSK3β or (B) phospho-FoxO3A and FoxO3A. Equal protein loading was assessed by blotting membranes with an antibody against tubulin. (C) Graphical presentations summarizing the effects of the different Jnk siRNAs vs siGFP in cytokine-treated cells; control values are set to 1. The data are the means±SD of three independent experiments. (**P<0.01) for cyto-Mix-siGFP vs cyto-Mix-si1, and cyto-Mix-si2. (***p<0.001) for cyto-Mix-siGFP vs cyto-Mix-si3. Significant differences were obtained for phospho-GSK3β in si1, si2, and si3 vs siGFP (***p<0.001).