Inherent Interfacial Mechanical Gradients in 3D Hydrogels Influence Tumor Cell Behaviors
Figure 4
OSU-2 cell behaviors as a function of observation plane in 40% (v/v) Matrigel.
(A) Schematic of cell-hydrogel constructs showing morphology observation at different “z” planes. (B) OSU-2 cell area. Representative cell morphologies are shown in the insets. As a result of surface roughness, zero height was set to the first plane of observed cells, which may not necessarily correspond to the substrate surface. (C) OSU-2 morphology at different heights. Representative heights are shown in the chart. Scale bar = 200 µm. (D) OSU-2 aspect ratio.