Mutagenesis and Functional Studies with Succinate Dehydrogenase Inhibitors in the Wheat Pathogen Mycosphaerella graminicola
Figure 7
Absence of a major oxidative stress related fitness penalty in M. graminicola homologous recombinant strains carrying Qp site mutations.
A: Respiratory growth of wild type and mutants in the presence of oxidative stresses. Upper panel, AE agar plate growth, middle panel: AE agar supplemented with 10 mM Paraquat, lower panel: AE agar plates were placed in a constant 100% atmosphere. All plates were incubated in same room at room temperature for 6 days. B: Sensitivity towards hydrogen peroxide in liquid AE media. Values presented are the averages from replicates within 3 individual experiments. C: Evaluation of mitochondrial ROS produced in vivo as determined with MitoSOX™ Red fluorescent indicator. Evaluations based on values from over 3 replicates within 3 individual experiments. +PQ corresponds to 1 mM Paraquat supplementation during the incubation period, +H2O2 correspond to 10 mM H2O2 supplementation during the incubation period. WT (IPO323), and homologous recombinant strains HRAV: SDHC_A84V, HRDG: SDHD_D129G, HRHL: SDHB_H267L, HRHR: SDHC_H152R, HRHY: SDHB_H267Y, HRIV: SDHB_I269V, HRNK: SDHC_N86K, HRRP: SDHB_R265P, HRSG: SDHC_S83G.