Cloning and Functional Analysis of FLJ20420: A Novel Transcription Factor for the BAG-1 Promoter
Figure 2
FLJ20420 mRNA expression in different tissues and cell lines.
Northern blot analysis of FLJ20420 mRNA expression in human normal tissues (A) and various tumor cell lines (B). FLJ20420 cDNA was used as a probe. The number on the left side indicates the size of the molecular mass markers. The same blot was then stripped and re-probed with human actin probe as an internal control. (C-D) Real-time PCR analysis of FLJ20420 and BAG-1 mRNA expression in lung cancer cell lines. NL9980 cells were used as a calibrator. The expression of FLJ20420 opposed BAG-1 expression in these cell lines. Error bars are representative of an average of 2 triplicate RT-PCR experiments. (E) Immunoblot analysis shows that BAG-1 protein expression corresponds to BAG-1 mRNA expression in the cell lines analyzed.