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Natural CD4+ T-Cell Responses against Indoleamine 2,3-Dioxygenase

Figure 3

CD4+ T cell responses against IDO.

(A), CD4+ T cell responses against IDOlong as examined by IFN-γ ELISPOT. PBMC from two malignant melanoma patients (left, middle) and one renal cell carcinoma patient (right) were stimulated once with IDOlong peptide before CD4+ T cells were isolated. The CD4+ cells were plated at 2×105 cells per well in duplicates with 104 DC either without or with the IDOlong peptide. As a control 104 DC were plated alone without T cells. The numbers of IFN-γ releasing cells were counted for each patient. (B), ex vivo ELISPOT analysis of CD4 T-cells isolated from three melanoma patients. CD4+ cells were isolated from PBMC from three different donors and directly plated at 2×105 cells per well in duplicates with 104 DC either without or with the IDOlong peptide. HLA-class II antibody were added to two additional wells with CD4 cells, dendritic cells and IDOlong peptide. The numbers of IFN-γ releasing cells were counted for each patient.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0034568.g003