A Knockout of the Tsg101 Gene Leads to Decreased Expression of ErbB Receptor Tyrosine Kinases and Induction of Autophagy Prior to Cell Death
Figure 6
Tsg101 knockout cells exhibit increased expression of LC3 and activated Cathepsin D.
Western blot analysis of LC3 as well as active Cathepsin D (CtsD) and its unprocessed precursors in Tsg101 knockout cells and their controls. Both types of cells were treated with bafilomycin A1 to assess the functionality autophagolysosomes. Note that treatment of Tsg101-deficient cells with this inhibitor, which prevents the acidification of lysosomes, reduces the level of processed Cathepsin D.