Epidermal Transglutaminase (TGase 3) Is Required for Proper Hair Development, but Not the Formation of the Epidermal Barrier
Figure 5
Protein extractability and transglutaminase activity in TGM3−/− tissue.
Proteins from tissue extracts of TGM3−/− and control skin were separated on an 8% SDS-PAGE and transferred to nitrocellulose. Probing with antibodies against involucrin revealed no alteration between the two genotypes, however the minor skin component trichohyalin showed an increased extractability (A). Protein extracts from wild type and TGM3−/− skin and tongue were analyzed for biotin-cadaverin incorporation into NN′dimethylcasein, coated onto microtitre plates. Zymogen activation of the protein extract with dispase induced activity only in extracts from wild type mice (B).