Divergent Roles of Salmonella Pathogenicity Island 2 and Metabolic Traits during Interaction of S. enterica Serovar Typhimurium with Host Cells
Figure 6
Effect of auxotrophic mutations on the function of the SPI2-encoded T3SS in HeLa cells.
HeLa cells were infected with S. Typhimurium WT or various mutant strains each harboring a plasmid for the expression of sseJ::HA at an MOI of 10. At 16 h after infection, the cells were fixed and the translocated SseJ-HA was detected by immuno-staining with monoclonal antibody against HA (red). Intracellular bacteria were detected by virtue of GFP expression (green) and host cell actin was labeled with phalloidin-Alexa647 (blue). Representative cells for each infecting strain are shown. Scale bars represent 10 µm.