Retinoids Regulate the Formation and Degradation of Gap Junctions in Androgen-Responsive Human Prostate Cancer Cells
Figure 5
Effect of 9-CRA, ATRA, and androgens on the formation of gap junctions in the presence and the absence of casodex.
LNCaP-32 cells, seeded in six well clusters containing glass cover slips, were allowed to grow to 70% confluence. Cells were then grown for additional 24 h in normal medium (NS), in charcoal-stripped, androgen-depleted medium alone (ST), in normal serum containing casodex (NS+CDX), and in androgen-depleted medium supplemented with MB (ST+MB), with MB and casodex (ST+MB+CDX), with 9-CRA (ST+CRA), 9-CRA and CDX (ST+CRA+CDX), with ATRA (ST+ATRA), and with ATRA and CDX (ST+ATRA+CDX). Degradation and subcellular localization of Cx32 were analyzed immunocytochemically as described in Materials and Methods. Note that GJs (green) are not degraded in cells treated with 9-CRA and ATRA both in the presence and absence of casodex whereas they are degraded in normal serum and androgen-depleted but MB supplemented medium containing casodex. E-cadherin is shown in red and the nuclei (blue) were stained with DAPI.