Mesenchymal Stem Cells Transfer Mitochondria to the Cells with Virtually No Mitochondrial Function but Not with Pathogenic mtDNA Mutations
Figure 5
Characteristics of the MSCs in conditioned media from 143B ρ0 cells.
A scanning electron microscopic examination showed a marked difference in the morphology of the MSCs in each conditioned medium (magnified at ×1,000; scale bars represent 10 µm). The MSCs in their own growth medium (A); after 24 h (B) and 48 h (C) in conditioned media, which were prepared by treating 143B ρ0 cells with uridine-free media for 48 h. The MSCs showed an increased migration in the conditioned media without uridine supplementation (D). A transmission electron microscopic examination was done after two days of Stage I coculture (magnified at ×9,000; scale bars represent 1 µm). There were intercellular contacts between ρ0 and the MSCs by either cytoplasmic process (E) or broad surface contact (F).