Chlorogenic Acid Stimulates Glucose Transport in Skeletal Muscle via AMPK Activation: A Contributor to the Beneficial Effects of Coffee on Diabetes
Figure 6
Increased AMPK activity and Akt phosphorylation in the absence of PI3K activation by CGA.
A: Myotubes were treated with 2 mmol/l CGA for various incubation periods up to 24 hours. B: Myotubes were treated with 2 mmol/l CGA for various incubation periods up to 24 hours. Whole cell lysate was immunoprecipitated with anti-AMPK α1/2. Immunoprecipitate was assayed against SAMS peptide in the presence of [γ-32P]ATP. Kinase activity was expressed as incorporated ATP/mg protein/minute. C: Myotubes were treated with vehicle, 100 nmol/l insulin or 2 mmol/l CGA. Whole cell lysate was immunoprecipitated with IRS-1 and immunoblotted for IRS-1 and p85 subunit of PI3K. D: Myotubes were treated with vehicle, 100 nmol/l insulin or 2 mmol/l CGA. Whole cell lysate was detected for p-Akt through immunoblotting. Results are the mean ± SE of three independent experiments. *P<0.05, **P<0.01 compared with controls.