Monodansylpentane as a Blue-Fluorescent Lipid-Droplet Marker for Multi-Color Live-Cell Imaging
Figure 3
MDH staining patterns are separated from known molecular markers for autophagic structures.
HepG2 cells transiently transfected with either EGFP-LC3B (top row), mcherry-atg5 (middle row), or mEos2-GABARAPL2 (bottom row) were stained with MDH and imaged. Under normal (not shown) and starvation conditions, EGFP-LC3B, TagRFP-atg5, or mEos2-GABARAPL2 puncta did not colocalize with MDH spots (right columns; merged). Scale bars, 10 µm.