Monitoring of Regulatory T Cell Frequencies and Expression of CTLA-4 on T Cells, before and after DC Vaccination, Can Predict Survival in GBM Patients
Figure 3
Decreased frequencies of Treg cells after DC vaccination are associated with extended survival.
PBMC from pre and post-DC vaccination time points were stained with an antibody cocktail that identifies Treg cell populations (CD3+CD4+CD25+CD127low). The ratio of post vaccination/pre-vaccination Treg cell frequencies from each patient was calculated and linked with the overall survival of each patient. (A) Flow cytometric analysis of Treg cell populations from a normal volunteer. (B) Representative FACS plots of Treg cell frequencies from two glioblastoma patients (7–799, 32–204) before and after DC vaccination.