The Homeodomain Protein Defective Proventriculus Is Essential for Male Accessory Gland Development to Enhance Fecundity in Drosophila
Figure 3
Localization of sperm and SP derived from dve mutant males.
(A) Female reproductive organs, uterus (ut), seminal receptacle (src), and spermatheca (spt), are visualized with DNA staining (magenta) at 30 min after mating. The Protamine B-GFP fusion proteins (ProB::GFP, green) are localized in the sperm nuclei. (B–G) Localization of control sperms (B–D) and dve-A mutant sperms (E–G) in reproductive organs of wild-type females at 30 min after mating. The dve-A mutant sperms are correctly localized to the sperm storage organs (src and spt). (H–J) Localization of the sex peptide-GFP fusion proteins (SP::GFP, green) in reproductive organs of wild-type females at 20–30 min after mating. Females mated with control (H), dve-A mutant (I), and dve KD (J) males are shown. Arrows indicate the sperm mass and arrowheads indicate the posterior mating plugs.