Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Endoplasmic Reticulum Stress-Sensing Mechanism Is Activated in Entamoeba histolytica upon Treatment with Nitric Oxide

Figure 3

Quantification of ER changes after nitric oxide treatment.

The nitric oxide induced changes were quantified using the iMARIS software. An average of 30 amoebas were analyzed per experiment. The total number of labeled compartments and the volume per labeled compartment were determined. The data show that both ER markers, KDEL and CRT, exhibit a similar change with a decrease in the volume of labeled vesicles and an increase in the number of vesicles present (A) . An unpaired t-test was performed and a statistically significant difference was found between treated and untreated samples for both ER markers (** p<0.05 and *** p<0.01). The calreticulin protein level was analyzed, 40,000 amoebas were loaded in each lane for each condition. An anti-actinin-2 antibody was used to control for equal loading of samples. No changes in the calreticulin protein levels were observed. Although there are changes in ER organization, the protein levels remain constant (B). Three independent experiments were conducted.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0031777.g003