Accelerated Identification of Proteins by Mass Spectrometry by Employing Covalent Pre-Gel Staining with Uniblue A
Figure 4
MS/MS fragmentation of an Uniblue A derivatized peptide.
The direct comparison of MS/MS fragmentation spectra of Uniblue A derivatized (top) versus un-labeled (bottom) peptide KVPQVSTPTLVEVSR displays significantly increased signal intensities for the derivatized N-terminal ions (a- and b- series, shown in red). The defined mass shift of 484.0399 m/z for modified residues allows the detection of fragments, which otherwise would be outside the measuring range (fragments a1-NH3 and b1).