Asiatic Acid Inhibits Liver Fibrosis by Blocking TGF-beta/Smad Signaling In Vivo and In Vitro
Figure 10
Knockdown of Smad7 from HSC-T6 cells prevents the inhibitory effect of AA on TGF-beta1-induced hepatic fibrosis in vitro.
A. Real-time PCR shows reduction of Smad7 mRNA by siRNA technique. B. Western blot analysis detects that knockdown of Smad7 from HSC-T6 cells results in a loss of AA (20 uM)-induced inhibition of TGF-beta1 (1 ng/ml)-mediated collagen I and a-SMA expression at 24 h. Data represent mean ± SEM for at least 3 independent experiments. *p<0.05, **p<0.01, ***p<0.001 versus control; ###p<0.001 versus TGF-beta1, DMSO, and control vector (P-super)-treated cells.