Heterokaryon Incompatibility Is Suppressed Following Conidial Anastomosis Tube Fusion in a Fungal Plant Pathogen
Figure 5
Heterokaryotic colony derived from a mixture of conidia from two incompatible strains that had undergone CAT fusion.
(A) Nuclear distribution in the heterokaryotic colony was not uniform and green and red nuclei formed clusters. Insets show magnified images of nuclei with corresponding numbers in the lower magnification image. Yellow nuclei were present in the colony. The images of nuclei are shown as projections of z stacks of images with the green and red channels merged. (B) Another example of hyphae with nuclei of different colours. (C) A hypha undergoing apparent cell death, indicated by the red fluorescence dispersed throughout the cytoplasm (arrow) without any visible hyphal fusion nearby. Bars, 40 µm. (D) Subcultures of a heterokaryotic colony showing the formation of several distinct sectors. (E) Single conidial isolates exhibiting resistance to both Hyg and Phleo. Bar 15 mm.