Isolation and Characterization of Maize PMP3 Genes Involved in Salt Stress Tolerance
Figure 3
Expression patterns of the eight ZmPMP3 genes in CN165 under various stresses.
One-week old shoots and roots of maize seedlings, treated by 200 mM NaCl, 4°C, 30% PEG-6000, and 100 µM ABA, were harvested respectively. The qRT-PCR was performed to determine the relative expression levels of the eight genes under various treatment. The maize GAPDH gene was selected as internal control. Data from qRT-PCR experiments were analyzed according to the 2−ΔΔCT method. Data represents the average of three independent experiments ±SE. when the relative expression levels go up to 2 fold or down to 0.5 fold, the difference of the expression is regarded as significant (P value<0.05).