Rod and Cone Pathway Signalling Is Altered in the P2X7 Receptor Knock Out Mouse
Figure 1
PCR for the vesicular transporter for ATP, VNUT mRNA expression using laser microdissected retinal samples.
(A–B) The outer nuclear layer (ONL) region targeted (A) and collected (B) for capture of photoreceptor mRNA. (C–D) The inner retinal region targeted (C) and collected (D) for capture of inner nuclear layer (INL) and ganglion cell layer (GCL) mRNA. E) RT-PCR was completed on laser microdissected samples from two WT retinas for both photoreceptor (PRs; 1&2) and INL/GCL (3&4) samples. Rhodopsin mRNA was present in photoreceptor samples and not INL/GCL sample, while Thy1 mRNA was present in INL/GCL samples and not the photoreceptor sample, confirming sample purity. (F) The mRNA for the vesicular transporter for ATP, VNUT was present in the retina in both the photoreceptor and the INL/GCL samples.